The effect of glycerol on the activity of betaglucosidase. Pdf pathogenicity of lasiodiplodia theobromae and fusarium. Influence of culture media, temperature and lightdarkness on. Morphological and molecular data reveal cryptic speciation. Postharvest treatment of fruit with hot water or fungicides can reduce the development of this disease.
Lasiodiplodia theobromae in the root rot disease complex of rice. Effect of mineral nutrition on anthracnose disease by. However, with this new development of severe dieback, similar to that described recently on other tree crops such as mango and kumquat in other countries khanzada et al. The fungus is commonly known as botryodiplodia theobromae pat. Frontiers biological control of mango dieback disease. Mycclial fragments were observed in the scales and the fungus was isolated in cycloheximide free culture media.
Archives of phytopathology and plant protection,112. Molecular characterization of cocoa, mango, banana and yam. Among these isolates, 19 12 sa and 7 nsa showed antagonistic. Lasiodiplodia theobromae, test their virulence and evaluate different fungicides to find out effective ones for.
Botryodiplodia theobromae, a common rot fungus, causes serious economic losses in cultivation of many different. On rare occasions it has been found to cause fungal keratitis. Botryodiplodia theobromae is a cosmopolitan and diverse species. As disease agent, pathogen is encountered in its anamorph state, named as lasiodiplodia. Lasiodiplodia theobromae is a common pathogen that occurs on a large number of hosts in the tropics and subtropics. The rot fungus botryodiplodia theobromae strains cross infect cocoa. Identification and pathogenicity of lasiodiplodia theobromae. Percentage rot of banana fruits was found to be maximum at 25 c and 30.
Direct microscopy analysis of the bronchoalveolar lavage specimens showed septate hyphae. Pdf morphological and molecular data reveal cryptic. Lasiodiplodia theobromae is a plant pathogen with a very wide host range. Botryodiplodia theobromae was isolated from the bark samples. Febs 18452 febs letters 407 1997 105110 partial purification and characterization of a jasmonic acid conjugate cleaving amidohydrolase from the fungus botryodiplodia theobromae silvia c. Metabolites produced by botryodiplodia theobromae synonym lasiodiplodia theobromae possessing plant growth regulating activities were shown to be the 7epimer of jasmonic acid and its derivatives. Their maturation is slow, and involves increased deposition of electronopaque material in the outer region of the original wall of the conidium and the appearance of an electrontransparent interior secondary wall. The purification and properties of extracellular betaglucosidase from botryodiplodia theobromae pat.
In addition, botryodiplodia theobromae has a wide host range in some tropical fruit crops such as mango, mangosteen, banana, pineapple and. References this ascomycotarelated article is a stub. Botryodiplodia theobromae, the anamorph of botryosphaeria rhodina berk. Plurivorous, on approximately 500 host plants 30, 181. Morphological and molecular characterization of lasiodiplodia theobromae from various banana cultivars causingcrown rot disease in fruits. A partial list of the recorded hosts includes citrus. Leaf and tuber analysis were carried out to determine effect of bounty fertilizer on mineral levels, phytochemical, phenol. The fungus has wide range of plant hosts and known to cause yield losses up to 80% especially on cash and food crop farms. Dieback due to lasiodiplodia theobromae, a new constraint to. Read culture conditions for jasmonic acid and biomass production by botryodiplodia theobromae in submerged fermentation, process biochemistry on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Evaluation of jasmonic acid production by lasiodiplodia theobromae under submerged fermentation kavitha mary jackson, marimuthu ponnusamy and sivakumar uthandi department of agricultural microbiology, tamil nadu agricultural university, coimbatore 641 003, tamil nadu, india corresponding author a b s t r a c t coconut and coco medium.
The objective of this study was to identify species of lasiodiplodia associated with dieback and stemend rot of. Metabolites produced by botryodiplodia theobromae synonym lasiodiplodia theobromae. Yoshinori asakawa, yoshiaki noma, in comprehensive natural products ii, 2010. Pdf morphological and molecular data reveal cryptic species. Tubers were incubated for seven days only with botryodiplodia theobromae because of rapid deterioration in a crd experiment of two treatments the two markets, lagos and abeokuta and seven replicates.
Dieback caused by the fungus lasiodiplodia theobromae is an important disease on mango plantations in the united arab emirates uae. Pathogenic association and management of botryodiplodia theobromae in guava orchards at sheikhupura district, pakistan. Corms that were devoid of petioles and shoot material, and which were scraped and stored at ambient conditions rapidly lost weight, as much as. Citrus is one of the most important fruit crops in florida and in the world. Botryodiplodia theobromae an overview sciencedirect topics. In the mississippi delta, botryodiplodia theobromae placed beneath the bark of sycamore trees incited cankers on 99% of trees inoculated in july, and on 66% of those inoculated in september. This fungus attacks more than 280 species of plants in different parts of the world domsch et al. In this study, 53 actinobacterial isolates were obtained from mango rhizosphere soil in the uae, of which 35 66% were classified as streptomycetes sa and 18 34% as nonstreptomycetes nsa. Business, international biological pest control methods diseases and pests guavas safety and security measures pests biological control plantpathogen relationships control. Filtrates from cultures of different ages of botryodiplodia theobromae pat.
Extensive tests of seed collected from pods on infected. Botryodiplodia theobromae may not be the main causal organism of this disease. However, sutton 5 has adopted the name lasiodiplodia theobromae griff. Serangan parah penyakit dapat menyebabkan kematian apabila tidak dikendalikan. Lasiodiplodia theobromae wikispecies, free species directory. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. Since the introduction of cocoa theobroma cacao in cameroon in 1886, the main disease has been phytophthora pod rot. Germination of conidia of botryodiplodia theobromae in. This finding is important in the study of management options for future prevention and.
His condition was complicated by enterococcus faecium peritonitis. Conclusion depending on the technique adopted, each of t. A dematiaceous mold was recovered and identified as l. Botryodiplodia stem end rot of mango and its control. Calcium nitrate fertilizer effect on anthracnose disease caused by colletotrichum gloeosporioides penz. It is a windborne, leafpenetrating, vascular pathogen, and may have evolved. Botryodiplodia theobromae, was most commonly associated with dieback and was consistently isolated from various tissues twigs, bark, vascular tissue and fruits of affected plants. Also isolated from ulcerated human cornea, lesions on nail and subcutaneous tissue. Evaluation of jasmonic acid production by lasiodiplodia. The fungus is found throughout the tropics and subtropics. Lasiodiplodia theobromae in citrus fruit diplodia stem. This disease has been reported in hawaii and india only, but unidentified botryodiplodia spp.
Botryodiplodia theobromae and diplodia natalensis is an economically important postharvest fruit decay that occurs on all types of citrus grown in florida and other hot, humid tropical and subtropical regions in the world. This study investigated infection and weight loss in market samples of ipomea batatas in relation to their phenol and calcium content which confer resistance to infection in plants. Learn about botryodiplodia theobromae see lasiodiplodia about us. Text is available under the creative commons attributionsharealike license. Botryodiplodia theobromae, dioscorea rotundata, tissue rot, yam introduction a great magnitude of losses of tuber crops resulting from many causes pathological and nonpathological in storage has been reported throughout the world noon, 1978, ricci et al. It is also a confirmation of the pathogenicity of l.
The conidia of botryodiplodia theobromae are initially hyaline and nonseptate and may be released from pycnidia in this condition. Botryodiplodia theobromae, is the major causal organism of soursop fruit. Postharvest diplodia stemend rot develops from latent. Inoculation tests were performed and the pathogenicity of the organism on its host was confirmed. Mango dieback and gummosis in sindh, pakistan caused by. It was also found that injury to the fruits enhanced the chances for infection. Induced sporulation of botryodiplodia theobromae pat. Read taxol from botryodiplodia theobromae bt 115an endophytic fungus of taxus baccata, journal of biotechnology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Morphological, pathological and molecular variability in. Lasiodiplodia theobromae pneumonia in a liver transplant.
The pathogenicity test showed that the isolates of lasiodiplodia theobromae, caused stem canker and needle blight on araucaria heterophylla. It causes rotting and dieback in most species it infects. There was an invariable association of lasiodiplodia theobromae with aerial as well as underground parts. Lasiodiplodia theobromae is a phytopathogenic fungus causing gummosis, a threatening disease for cashew plants in brazil. Red globe plants confirmed the pathogenicity of the isolates and revealed differences in virulence among the isolates tested. Lasiodiplodia theobromae name synonyms botryodiplodia ananassae sacc. The hydrolysis of onitrophenyl betadglucopyranoside by the highmolecularweight betaglucosidase betadglucoside glucohydrolase, ec 3.
Muhammad ali khanzada, abdul mubeen lodhi, and saleem shahzad, pest and disease research lab, department of botany. Botryodiplodia theobromae grew and sporulated best at 28c on media amended with ripe mangoextract and dextrose or sucrose. Botryodiplodia theobromae causes stem rot disease of citrus. Lasiodiplodia theobromae was identified based on morphological characters as described by punithalingam 1976. Colonization of cashew plants by lasiodiplodia theobromae ainfo. Business, international butt rots observations fungi, pathogenic diseases and pests kenaf plant pathogenic fungi. Morphological and molecular data reveal cryptic speciation in lasiodiplodia theobromae. Calcium nitrate fertilizer effect on anthracnose disease. Proceedings of an international workshop held at chiang mai, thailand, 1821 may 1997. It is a common post harvest fungus disease of citrus known as stemend rot. This page was last edited on 14 august 2019, at 00. However, the cankers are favored by high temperatures.
Kinetic analysis of the mechanism of action of betaglucosidase from botryodiplodia theobromae pat. A causal pathogen of black rot disease on kenaf seeds in malaysia. Report by international journal of agriculture and biology. The biology of botryodiplodia cankers in oaks is poorly known. Materials and methods field layout the field plot has been monocropped to dioscorea. Thirteen isolates of botryodiplodia theobromae collected from pear varieties grown in various regions of punjab were studied for morphological, pathological. Pdf species of lasiodiplodia associated with mango in brazil. Desapping placing the stem end of newly harvested fruit into the soil or turf beneath trees should be avoided since infection by l. Diplodia stemend rot caused by lasiodiplodia theobromae synonyms. Introduction botryodiplodia theobromae pathogen fungi grow on diverse. Some pathological studies on botryodiplodia theobromae pat. Botryodiplodia theobromae is a virulent plant pathogen commonly found in the tropics and subtropics. Control of botryodiplodia theobromae rot of white yam.
Morphological, pathogenic and molecular characterization of. We report a case of lasiodiplodia theobromae pneumonia in a patient who died 14 days after cadavericliver transplantation. Plant pathogen causing many tropical fruit diseases including mango twig blight and mango stem rot. Pdf the rot fungus botryodiplodia theobromae strains cross infect. Kaur2 1division of plant pathology, sherekashmir university of agricultural sciences and technology of kashmir, shalimar, srinagar, jammu and kashmir, india. Old or inactive cankers appear sunken and are surrounded by callus tissues. Pathogenic association and management of botryodiplodia. Sycamore canker caused by botryodiplodia theobromae. First report of lasiodiplodia theobromae causing needle. A description is provided for botryodiplodia theobromae. Pdf mango plantations in different areas of sindh were found to suffer from a decline disease showing symptoms of. Five cellulases c1, c2, c3, c4 and c5 were found, and their molecular. Dieback due to lasiodiplodia theobromae, a new constraint. It also infects biancaea sappan, a species of flowering tree also known as sappanwood.
After 5 days, the mycelium was homogenized with distilled water and used for inoculation of the main culture. Lasiodiplodia theobromae and the roles of insects in dispersal of the fungi. Kuntze 1898 botryodiplodia ulmicola is an ascomycete fungus that is a plant pathogen. But, it is an opportunistic pathogen and becomes more virulent in combination with others fungi, i. Mango dieback and gummosis in sindh, pakistan caused by lasiodiplodia theobromae. The fungal pathogen lasiodiplodia theobromae has been associated with. Partial purification and characterization of a jasmonic. This study aimed at establishing genetic diversity of b. Mango mangifera indica is a major tropical fruit species cultivated in brazil. Lasiodiplodia theobromae as a cause of keratomycoses. Botryodiplodia theobromae and its association with musa. Pdf morphological, pathological and molecular variability in. The active site of betaglucosidase from botryodiplodia. Emerging resistance against different fungicides in lasiodiplodia theobromae as the cause of mango dieback in pakistan ateeq ur rehman1, ummad ud din umar 1, syed atif hasan naqvi1, munaza rana latif, sajid aleem khan2, muhammad tariq malik3 and shoaib freed4 1 department of plant pathology, bahauddin zakariya university, multan, pakistan.
Stotzky with 7 figures botryodiplodia theobromae pat. The fungus botryodiplodia theobromae strain d 72 1 isolated from cuban oranges was maintained and pre. The fungus was also strongly inhibited by benomyl, with radial growth reduced. Tujuan penelitian adalah mengetahui patogenisitas dan peran toksin dari tiga isolat b. This disease can lead to large losses as it can attack plants from the nursery until the time of production.
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